发明名称 |
Vibrio cholerae lipoprotein 15 (Lp15) variants as anti-interference additive in TpN17-based immunoassays for detection of anti-Treponema antibodies |
摘要 |
The invention relates to a method for detecting antibodies against the TpN17 antigen of Treponema pallidum in an isolated sample wherein a peptide sequence of Vibrio cholerae lipoprotein 15 ( Vc Lp15) or a partial sequence thereof is used as a reagent for reduction of interference , i.e. for minimizing false positive results. In addition the invention relates to fusion polypeptides comprising a Vc Lp15 peptide sequence and a chaperone, to their use as an additive in an immunoassay for said reduction of interferences and for minimizing false positive results and to a reagent kit for detecting antibodies against Treponema pallidum antigens in an isolated sample comprising a TpN17 antigen and said Vc Lp15-chaperone fusion polypeptide. |
申请公布号 |
EP2827147(B1) |
申请公布日期 |
2017.03.01 |
申请号 |
EP20140177199 |
申请日期 |
2014.07.16 |
申请人 |
Roche Diagnostics GmbH;F. Hoffmann-La Roche AG |
发明人 |
Faatz, Elke;Schaarschmidt, Peter;Schmitt, Urban;Scholz, Christian |
分类号 |
G01N33/53;G01N33/571 |
主分类号 |
G01N33/53 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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