| 发明名称 |
Synthesis of long fish probes |
| 摘要 |
A method comprising: synthesizing a set of overlapping oligonucleotides that comprises probe sequences that hybridize to unique sequences in a chromosome, assembling the overlapping oligonucleotides in a way that produces one or more double stranded polynucleotides that each comprises multiple probe sequences, labeling the one or more double stranded polynucleotides to produce one or more labeled probes, and hybridizing the labeled probes to an intact chromosome, in situ, is provided. |
| 申请公布号 |
US9580746(B2) |
申请公布日期 |
2017.02.28 |
| 申请号 |
US201414146216 |
申请日期 |
2014.01.02 |
| 申请人 |
Agilent Technologies, Inc. |
发明人 |
Leproust Emily Marine;Chen Siyuan;Ruvolo Michael |
| 分类号 |
C12Q1/68;G06F19/20 |
主分类号 |
C12Q1/68 |
| 代理机构 |
|
代理人 |
|
| 主权项 |
1. A method comprising:
(a) synthesizing a set of overlapping oligonucleotides that comprises probe sequences that hybridize to unique sequences in a chromosome; (b) assembling the overlapping oligonucleotides in a way that produces one or more double stranded polynucleotides that each comprises multiple probe sequences, wherein the contiguous nucleotide sequence of each of said one or more double stranded polynucleotides is at least 95% identical to a sequence of said chromosome; (c) labeling the one or more double stranded polynucleotides to produce one or more labeled probes; and (d) hybridizing said labeled probes to an intact chromosome that comprises repeat sequences, in situ. |
| 地址 |
Santa Clara CA US |
