Method for producing extracellular proteins from genus <i>Tepidimonas</i>

摘要

The disclosure provides a method for producing extracellular proteins from genus Tepidimonas, including: performing a fermentation culturing to a bacteria of genus Tepidimonas with a culturing medium to form a fermented liquid, wherein the composition of the culturing medium includes a carbon source which is an organic acid, selected from a group consisting of acetate, lactate and butyrate; a nitrogen source selected from a group consisting of (NH4)2SO4, NH4NO3, NH4Cl and urea; phosphate; carbonate; MgCl2; yeast extract; and trace elements, and wherein the gas feeding rate for the fermentation culturing is about 0-0.1 vvm; and after the fermentation culturing is completed, collecting the fermented liquid, wherein the fermented liquid contains extracellular protein secreted from the bacteria of genus Tepidimonas.

主权项

1. A method of producing extracellular proteins from genus Tepidimonas, comprising:
fermenting a Tepidimonas in a fermenter with a culture medium in an air feeding type to obtain a suspension; and wherein the culture medium comprises: a carbon source which is an organic acid, selected from a group consisting of acetate, lactate and butyrate; a nitrogen source selected from a group consisting of ammonium sulfate ((NH4)2SO4), ammonium nitrate (NH4NO3), ammonium chloride (NH4Cl) and urea; a phosphate; a carbonate; magnesium chloride (MgCl2); a yeast extract; and trace elements; collecting the suspension, the suspension comprising the extracellular proteins from genus Tepidimonas, wherein the air feeding type is about 0.001-0.1 vvm air feeding rate.