主权项 |
1. A recombinant polypeptide comprising at least one PUF RNA-binding domain, wherein the PUF RNA-binding domain comprises at least one RNA base-binding motif having formula X 1X2X3X4X5X6X7X8X9X10X11 wherein
X1 is glutamine (Q), X2 is histidine (H); X3 is glycine (G); X4 is selected from the group including glycine (G), alanine (A), serine (S), threonine (T) and cysteine (C); X5 is arginine (R); X6 is phenylalanine (F); X7 is isoleucine (I); X8 is arginine (R); X9 is leucine (L); X10 is lysine (K); and X11 is leucine (L); or X1 is valine (V); X2 is phenylalanine (F); X3 is glycine (G); X4 is selected from the group including glycine (G), alanine (A), serine (S), threonine (T) and cysteine (C); X5 is tyrosine (Y); X6 is valine (V); X7 is isoleucine (I); X8 is arginine (R); X9 is lysine (K); X10 is phenylalanine (F); and X11 is phenylalanine (F); or X1 is methionine (M); X2 is tyrosine (Y); X3 is glycine (G); X4 is selected from the group including glycine (G), alanine (A), serine (S), threonine (T) and cysteine (C); X5 is arginine (R); X6 is valine (V); X7 is isoleucine (I); X8 is arginine (R); X9 is lysine (K); X10 is alanine (A); and X11 is leucine (L); or X1 is glutamine (Q); X2 is asparagine (N); X3 is glycine (G); X4 is selected from the group including glycine (G), alanine (A), serine (S), threonine (T) and cysteine (C); X5 is histidine (H); X6 is valine (V); X7 is valine (V); X8 is arginine (R); X9 is lysine (K); X10 is cysteine (C); and X11 is isoleucine (I); or X1 is proline (P); X2 is tyrosine (Y); X3 is glycine (G); X4 is selected from the group including glycine (G), alanine (A), serine (S), threonine (T) and cysteine (C); X5 is arginine (R); X6 is valine (V); X7 is isoleucine (I); X8 is arginine; (R); X9 is arginine (R); X10 is isoleucine (I); and X11 is leucine (L); or X1 is glutamine (Q); X2 is tyrosine (Y); X3 is glycine (G); X4 is selected from the group including glycine (G), alanine (A), serine (S), threonine (T) and cysteine (C); X5 is tyrosine (Y); X6 is valine (V); X7 is isoleucine (I); X8 is arginine; (R); X9 is histidine (H); X10 is valine (V); and X11 is leucine (L); or X1 is lysine (K); X2 is phenylalanine (F); X3 is alanine (A); X4 is selected from the group including glycine (G), alanine (A), serine (S), threonine (T) and cysteine (C); X5 is asparagine (N); X6 is valine (V); X7 is valine (V); X8 is arginine; (R); X9 is lysine (K); X10 is cysteine (C); and X11 is valine (V); or X1 is glutamine (Q); X2 is tyrosine (Y); X3 is alanine (A); X4 is selected from the group including glycine (G), alanine (A), serine (S), threonine (T) and cysteine (C); X5 is tyrosine (Y); X6 is valine (V); X7 is valine (V); X8 is arginine; (R); X9 is lysine (K); X10 is methionine (M); and X11 is isoleucine (I). |