METHOD FOR ESTIMATING A QUANTITY OF PARTICLES DIVIDED INTO CLASSES, USING A CHROMATOGRAM

摘要

The invention is a method for estimating a quantity or a concentration of particles using a detector disposed at the exit of a chromatography column. The estimation is carried out on the basis of a selection of a plurality of retention times within the histogram, each retention time being associated with an individual particle. The method aims to classify each retention time into one or more classes, each class being representative of a species of particles. The method can include an estimation of the number of classes.

主权项

1. A Method for estimating a quantity of particles present in a sample, comprising:
a) passing the sample through a chromatography column, the said column comprising a detector capable of detecting the said particles, the detector delivering a chromatogram representing the number of particles detected as a function of a retention time, representative of the time spent by each particle in the column; b) constituting a list comprising a plurality of retention times, each retention time being associated with an individual particle, the said list being established by random sampling from the said chromatogram; c) carrying out a classification of each retention time on the said list according to a plurality of classes, with each class there being associated an a priori distribution of the retention times defined by parameters, the said parameters being distributed according to a predetermined base distribution; d) estimating a quantity or a proportion of particles whose retention time is classified according to at least one of the said classes defined during the step c).