Expression vector comprising a polynucleotide encoding a modified glutamine synthetase and a method for preparing a target protein employing the same

摘要

The present invention relates to a vector comprising a polynucleotide encoding a modified glutamine synthetase (GS), and a method for preparing a target protein employing the same. More particularly, the present invention relates to a modified GS having an increased sensitivity to a glutamine synthetase (GS) inhibitor, a polynucleotide encoding the modified GS, a vector comprising the polynucleotide, a transformant comprising the vector, and a method for preparing a target protein using the transformant.

主权项

1. A vector for expression of a target protein, comprising a polynucleotide encoding a modified glutamine synthetase (GS), wherein glycine (Gly, G) at position 299 of a glutamine synthetase having the amino acid sequence shown in SEQ ID NO. 4 is substituted with arginine (Arg, R) and a gene encoding the target protein.