发明名称 |
Ultrasound mediated delivery of substances to algae |
摘要 |
Embodiments provided herein generally relate to methods and materials for ultrasound-mediated introduction of exogenous substances into microorganisms. Some embodiments relate to methods of introducing an exogenous material into a microorganism such as an algae. The methods can include, for example, providing a microorganism (e.g., algae); providing a population of bubbles that comprise one or more nucleic acid molecules and/or one or more polypeptide or one or more protein molecules; contacting the population of bubbles with the algae; applying ultrasound to the bubbles and algae with sufficient energy to cavitate one or more of bubbles comprising the one or more nucleic acid molecules in proximity to the algae; and maintaining the algae and the one or more burst bubbles comprising the one or more nucleic acid molecules in contact for a period of time sufficient to permit entry of at least one nucleic acid molecule into the algae. |
申请公布号 |
US9567597(B2) |
申请公布日期 |
2017.02.14 |
申请号 |
US201314400136 |
申请日期 |
2013.05.11 |
申请人 |
UNIVERSITY OF HAWAII |
发明人 |
Liaw Eric;Walton Chad B.;Shohet Ralph V. |
分类号 |
C12N15/82;C12M1/42;C12N13/00;C12N15/87;C12M1/00 |
主分类号 |
C12N15/82 |
代理机构 |
Knobbe, Martens, Olson & Bear LLP |
代理人 |
Knobbe, Martens, Olson & Bear LLP |
主权项 |
1. A method of introducing an exogenous material into an algae, comprising:
providing an algae; providing a population of bubbles that comprise one or more nucleic acid molecules and/or one or more polypeptide or protein molecules; contacting the population of bubbles with the algae; subsequently applying ultrasound to the bubbles and algae with sufficient energy to cavitate one or more of bubbles comprising the one or more nucleic acid molecules in proximity to the algae; and maintaining the algae and the one or more burst bubbles comprising the one or more nucleic acid molecules in contact for a period of time sufficient to permit entry of at least one nucleic acid molecule into the algae. |
地址 |
Honolulu HI US |