Dielectric spectroscopy methods and apparatus

摘要

Methods and apparatus are disclosed for correcting measurements received by applying a frequency-varying signal with a measuring device (e.g., a permittivity probe) to a population of living cells (e.g., contained in a bioreactor) and correcting measurement divergences using data acquired using an alternate analytical method (e.g., trypan blue exclusion). In one example, a method comprises receiving electrical property data for a first population of cells, the data obtained by applying a first frequency-varying signal to the population with a measuring device, receiving biological property data obtained using an alternate analytical technique, generating at least one value representative of the frequency dependence of the electrical property data, and determining a relationship between the biological property data and the representative value. In some examples, measurements of apoptosis are predicted using the electrical property data.

主权项

1. A method for determining a biological property of cells suspended in a liquid media, the method comprising:
applying an electrical signal to said cells suspended in said liquid media at two or more signal frequencies to generate electrical property data; receiving biological property data obtained using an alternate analytical technique; generating at least one value representative of frequency dependence of said electrical property data; determining a first relationship between said biological property data and said electrical property data; determining a second relationship between (1) said value representative of the frequency dependence of said electrical property data and (2) a divergence between a magnitude of said biological property data and a magnitude of said electrical property data; determining a calibration function by combining said first relationship and said second relationship; by a computer, calculating said calibration function with subsequent electrical property data to determine said biological property of said cells; and adjusting one or more environmental conditions of said liquid media in response to determining said biological property of said cells.