THERMOCYCLING SYSTEM, COMPOSITION, AND MICROFABRICATION METHOD

摘要

A system and method of manufacture for the system, comprising a set of heater-sensor dies, each heater-sensor die comprising an assembly including a first insulating layer, a heating region comprising an adhesion material layer coupled to the first insulating layer and a noble material layer, and a second insulating layer coupled to the heating region and to the first insulating layer through a pattern of voids in the heating region, wherein the pattern of voids in heating region defines a coarse pattern associated with a heating element of the heating region and a fine pattern, integrated into the coarse pattern and associated with a sensing element of the heating region; an electronics substrate configured to couple heating elements and sensing elements of the set of heater-sensor dies to a controller; and a set of elastic elements configured to bias each of the set of heater-sensor dies against a detection chamber.

主权项

1. A system for thermocycling biological samples within detection chambers comprising:
a set of heater-sensor dies, each heater-sensor die in the set of heater-sensor dies comprising:
an assembly including a first insulating layer, a heating region comprising an adhesion material layer coupled to the first insulating layer and a noble material layer coupled to the adhesion material layer, and a second insulating layer coupled to the first insulating layer through a pattern of voids in the heating region,wherein the pattern of voids in the heating region defines a coarse pattern, comprising a global morphology at a first scale and associated with a heating element of the heating region, and a fine pattern, comprising a local morphology at a second scale smaller than the first scale, integrated into the coarse pattern and associated with a sensing element of the heating region.