METHOD OF BIOSYNTHESIZING FUNCTIONAL LIPID USING NANO-MAGNETICALLY IMMOBILIZED ENZYME FOR CONTINUOUS TARGETED CATALYSIS OF PHOSPHOLIPID IN SOYBEAN OIL

摘要

Provided is a method of biosynthesizing a functional lipid using a nano-magnetically immobilized enzyme for continuous targeted catalysis of a phospholipid in soybean oil, comprising: applying nano-magnetically immobilized phospholipid enzymes in a three-phase magnetic fluidized bed, as a solid phase, such that the enzymes are distributed in a magnetically stable fluidized state; using soybean oil as a continuous flow phase, wherein the soybean oil passes through different types of magnetic fluidized bed reactors of nano-magnetically immobilized enzymes, and contacts and catalyzes a phospholipid without requiring mechanical stirring, generating a corresponding product; immobilizing the nano-magnetically immobilized lipase in a three-phase magnetic fluidized bed; through adding a phytosterol, esterifying FFA in a substrate with the phytosterol to form a phytosterol ester, thus changing a conventional removal step to a process of biosynthesizing a functional lipid. In the finished product, the content of DAG is approximately 1.65%, the content of phytosterol ester is approximately 1.16%, the content of phosphorous is 9.29 mg/kg and the esterification rate of the phytosterol ester is 93.4%. Continuous production is realized by re-using two enzymatic hydrolysis processes.