| 摘要 |
The present invention relates to the use of a control marker for implementing analysis methods on spots, in particular in the context of multiplex analyses. The present invention thus relates to solid supports containing said control marker, their preparation method and their use in analysis methods. The present invention makes it possible to verify the presence, location and/or integrity of the spots at the end of the analysis method, and thus to secure the obtained results while guaranteeing that the yielded result indeed results from a present, intact and localized spot. |
| 主权项 |
1. A method for detecting at least one analyte in at least one sample comprising the following steps:
a) placing a sample to be analyzed in the presence of the spot(s) of the compartment of a solid support, said spot or at least one of said spots comprising at least one resistant control marker and at least one capture ligand of an analyte, b) placing at least one detection ligand of an analyte in the presence of the spot(s) of said compartment, said detection ligand of an analyte being coupled to a direct or indirect detection marker, c) when at least one detection ligand of an analyte is coupled to an indirect detection marker, placing a reporter of said indirect detection marker in the presence of the spot(s) of said compartment, d) when the reporter used in step c) is coupled to an indirect detection marker, placing a reporter of the indirect detection marker coupled to said reporter used in step c) in the presence of the spot(s) of said compartment, e) detecting at least one signal produced by at least one resistant control marker in said compartment, f) defining at least one reading grid from the location of at least one signal detected in step e), g) optionally, detecting a signal produced by at least one detection marker of a detection ligand of an analyte, and h) optionally, reading the signal detected in step g) on the reading grid defined in step f). |
