ACCURATE DETECTION OF RARE GENETIC VARIANTS IN NEXT GENERATION SEQUENCING

摘要

The invention relates to a method for analyzing a target nucleic acid fragment, comprising generating a first strand using one strand of the target as a template by primer extension, using a first oligonucleotide primer which comprises, from 5′ to 3′, an overhang adaptor region, a primer ID region and a target specific sequence region complementary to one end of the target fragment; optionally removing non-incorporated primers; amplifying the target from the generated first strand to produce an amplification product; and detecting the amplification product. Also disclosed are unique primers useful for such target analysis methods.