ENHANCEMENT OF RECOMBINANT PROTEIN EXPRESSION WITH COPPER

摘要

The present invention provides a novel use of copper (cupric ion) for improved cell expression of recombinant proteins, particularly coagulation proteins such as recombinant Factor VIII, B Domain Deleted recombinant Factor VIII, recombinant Factor IX and rFVII or rFVIIa. The use of such cell culture supplement results in higher productivity and robustness of the manufacturing process. This invention results in improvements in cell expression and product stability.