METHODS AND COMPOSITIONS FOR DISCRIMINATION BETWEEN CYTOSINE AND MODIFICATIONS THEREOF, AND FOR METHYLOME ANALYSIS

摘要

Compositions and methods are provided for discrimination between cytosine and modifications thereof using cytidine deaminases. Variants of wild type cytidine deaminases are described which show reduced bias with respect to adjacent nucleotides upstream of the cytosine. The methods provide a rapid and convenient use of enzymes to obtain methylomes.