发明名称 |
QUANTIFICATION OF TARGET NUCLEIC ACID USING COMMON INTERNAL CONTROL |
摘要 |
The present invention relates to a method for quantifying a target nucleic acid sequence by use of a common internal control. The present invention allows to determine an absolute initial amount of a target nucleic acid sequence with no use of a standard curve. The present invention amplifies not only a control reaction mixture comprising a known-amount standard and an internal control but also a sample reaction mixture comprising an internal control identical to the internal control in the control reaction mixture and the target nucleic acid sequence to be quantified. In the present invention, a relative ratio of the amount of the standard and the amount of the target nucleic acid sequence is calculated by using the internal control and then an initial amount of the target nucleic acid sequence is determined by using a known amount of the standard. |
申请公布号 |
EP2992113(A4) |
申请公布日期 |
2017.02.01 |
申请号 |
EP20140791659 |
申请日期 |
2014.05.02 |
申请人 |
Seegene, Inc. |
发明人 |
CHUN, Jong Yoon;LEE, Young Jo |
分类号 |
C12Q1/68;C12N15/11 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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