发明名称 |
組換え脱アミドグリアジン抗原 |
摘要 |
The present invention provides a method for determining whether a subject is suffering from celiac disease by contacting a sample of bodily fluid from the subject, with an antigen formed from a gliadin fusion protein immobilized on a solid support. The gliadin fusion protein of the antigen includes a recombinant deamidated gliadin linked to a tag such as Glutathione-S transferase (GST) protein. The antigen is prepared by immobilizing on the solid support the gliadin fusion protein via the tag. The antigen can further include tissue Transglutaminase (tTG) cross-linked to the gliadin fusion protein. When tTG is present, the tTG and recombinant deamidated gliadin are mixed together prior to immobilization to the solid phase. |
申请公布号 |
JP6073940(B2) |
申请公布日期 |
2017.02.01 |
申请号 |
JP20150021121 |
申请日期 |
2015.02.05 |
申请人 |
バイオ−ラッド ラボラトリーズ,インコーポレイティド |
发明人 |
ワトキンス,マイケル アイ.;マー,グレゴリー エー.;ヤン,シャオユン;ブルール,リチャ−ド;シャン,ダミン;コールマン,パトリック エフ. |
分类号 |
C07K14/415;C07K19/00;C12N9/10;C12N15/09;C12Q1/48;G01N33/531;G01N33/543;G01N33/564 |
主分类号 |
C07K14/415 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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