| 摘要 |
A process for producing a cross-linked crystallized protein complex, which comprises: a first step of concentrating a crude protein derived from an animal or plant; a second step of encapsulating the protein in a gel, to thereby allow the protein to undergo air oxidation, and then extracting a protein complex from the gel; a third step of allowing the extracted protein complex to undergo crystallization and precipitation; and a fourth step of cross-linking the precipitated protein complex. Alternatively, by use of a fifth step of drying (FD) the obtained crosslinked crystallized protein complex, to thereby form a powder. As a result, there is provided an enzyme which is stable at room temperature storage, and has an activity in catalyzing an asymmetric oxidation reaction. That is, there is provided a useful material which enables an efficient enzyme-mimetic reaction under a mild condition. |
| 主权项 |
1. A protein complex containing at least a protein having at least one peak in a region of (1,085±50 cm−1) and a region of (1,411±50 cm−1), respectively, in FT-IR,
wherein the protein comprises a precipitated and cross-linked protein, wherein the protein complex has an activity in catalyzing an asymmetric oxidation reaction, which selectively gives one enantiomer of a substrate racemic alcohol, wherein the protein complex has an active domain of a redox reaction, wherein the substrate racemic alcohol is selected from secondary alcohols having a benzene or naphthalene skeleton; or secondary alcohols having an alkyl chain and having no benzene or naphthalene skeleton, wherein the protein complex has a group, which has been produced by the air oxidation of the —SH group of cysteine, wherein the —SH group of cysteine has been derived from a crude protein from a water-soluble moiety derived from a plant, and wherein the plant is selected from grains selected from the group consisting of buckwheat, amaranth, rice, wheat, barley, corn, oats, rye, foxtail millet, barnyard millet, millet, adlay, and sorghum, and beans selected from the group consisting of adsuki beans, kidney beans, green peas, green beans, and soy beans. |
