Enzyme quantification

摘要

The invention generally relates to methods for quantifying an amount of enzyme molecules. Systems and methods of the invention are provided for measuring an amount of target by forming a plurality of fluid partitions, a subset of which include the target, performing an enzyme-catalyzed reaction in the subset, and detecting the number of partitions in the subset. The amount of target can be determined based on the detected number.

主权项

1. A method for quantifying enzyme molecules, the method comprising:
forming a plurality of fluid partitions comprising enzyme molecules and substrates in a microfluidic channel, wherein at least one substrate comprises a detectable label; conducting an enzymatic reaction in at least one of the fluid partitions, wherein the detectable label is released; and quantifying the enzyme molecules by determining a number of fluid partitions that released the detectable label.