Method for producing EPA-enriched oil and DHA-enriched oil

摘要

Alcoholysis of oils and fats containing EPA and DHA is performed by a lipase having substrate specificity for fatty acids having 18 carbons or less and in the presence of a reaction additive such as magnesium oxide; then the glyceride fraction is separated; alcoholysis of the glyceride fraction is performed by a lipase having substrate specificity for fatty acids having 20 carbons or less and in the presence of a reaction additive such as magnesium oxide; and EPA-enriched oil and DHA-enriched oil are simultaneously obtained.

主权项

1. A method for producing eicosapentaenoic acid-enriched oil and docosahexaenoic acid-enriched oil from oils and fats containing eicosapentaenoic acid and docosahexaenoic acid as constituent fatty acids, said method comprising:
a) a step wherein said oils and fats are subjected to alcoholysis accompanied by hydrolysis, by a lipase having substrate specificity for fatty acids having 18 carbons or less and in the presence of an aqueous alcohol and magnesium oxide as a reaction additive, and an eicosapentaenoic acid and docosahexaenoic acid-enriched glyceride fraction is obtained from the resulting reaction mixture, wherein water is added in the amount of 5% (v/v) to 20% (v/v) in terms of amount of the aqueous alcohol, and b) a step wherein said glyceride fraction is subjected to alcoholysis accompanied by hydrolysis, by a lipase having substrate specificity for fatty acids having 20 carbons or less and in the presence of an aqueous alcohol and magnesium oxide as a reaction additive, and the eicosapentaenoic acid-enriched ester fraction and docosahexaenoic acid-enriched glyceride fraction are separated from the resulting reaction mixture, wherein water is added in the amount of 5% (v/v) to 20% (v/v) in terms of amount of the aqueous alcohol; wherein the lipase used in step a) is a lipase obtained from a microorganism belonging to Alcaligenes sp. and the lipase used in step b) is a lipase obtained from a microorganism belonging to Thermomyces lanuginosus; and wherein the oils and fats used in step a) are fish oil.