Multiple arm apparatus and methods for separation of particles

摘要

The invention provides apparatus for separation of particles and methods for using the apparatus. In an embodiment, the apparatus includes three arms extending radially from a central reservoir, each arm being associated with a separation electrode. At least one on the arms includes a separation medium. Using a sequence of driving and mobility-changing voltages, target particles can be separated from closely related particles within a sample. For example, single point mutations can be resolved from a sample containing predominantly wild type nucleic acids.

主权项

1. An apparatus for separating particles comprising:
at least three electrodes circumferentially surrounding a central reservoir; a separation medium between at least one electrode and the central reservoir; an arm between each of the at least three electrodes and the central reservoir, at least one of the arms comprising the separation medium, wherein the arm comprising the separation medium comprises a loading reservoir adjacent the separation medium and the loading reservoir extends perpendicularly relative to the length of the separation arm; and a loading buffer chamber adjacent the separation medium and opposite the loading reservoir.