发明名称 |
METHODS OF AMPLIFYING NUCLEIC ACID SEQUENCES |
摘要 |
Methods are provided for nucleic acid amplification including contacting a double stranded nucleic acid with transposases bound to transposon DNA, wherein the transposon DNA includes a transposase binding site and an RNA polymerase promoter sequence, wherein the transposases/transposon DNA complex bind to target locations along the double stranded nucleic acid and cleave the double stranded nucleic acid into a plurality of double stranded fragments, with each double stranded fragment having the transposon DNA bound to each 5' end of the double stranded fragment, extending the double stranded fragments along the transposon DNA to make double stranded extension products having double stranded RNA polymerase promoter sequences at each end, contacting the double stranded extension products with an RNA polymerase to make a plurality of RNA transcripts of each double stranded extension product, reverse transcribing the RNA transcripts into single stranded copy DNA, fonning complementary strands to the single stranded copy DNA to form a plurality of double stranded DNA amplicons corresponding to each double stranded fragment. |
申请公布号 |
WO2017015075(A1) |
申请公布日期 |
2017.01.26 |
申请号 |
WO2016US42394 |
申请日期 |
2016.07.15 |
申请人 |
PRESIDENT AND FELLOWS OF HARVARD COLLEGE |
发明人 |
CHEN, Chongyi;XING, Dong;XIE, Xiaoliang Sunney |
分类号 |
C12Q1/68;C40B40/06;C40B50/00;C40B50/06;C40B50/10 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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