发明名称 |
Methods and Systems for Quantification Without Standard Curves |
摘要 |
A method for quantitation of biological material in a biological sample is provided. The method includes receiving amplification data from amplification of a first and a second reference sample and receiving amplification data from amplification of a biological sample. The method further includes determining an efficiency from the received amplification data from amplification of the first and second reference sample. The method includes determining a relative PCR efficiency for the biological sample. Next, the method includes determining a quantity of biological material in the biological sample using the relative PCR efficiency. |
申请公布号 |
US2017022552(A1) |
申请公布日期 |
2017.01.26 |
申请号 |
US201515113030 |
申请日期 |
2015.01.20 |
申请人 |
LIFE TECHNOLOGIES CORPORATION |
发明人 |
Gjerstad Carmen;Dong Shoulian |
分类号 |
C12Q1/68 |
主分类号 |
C12Q1/68 |
代理机构 |
|
代理人 |
|
主权项 |
1. A method for quantitation of biological material in a biological sample, the method comprising:
receiving amplification data from amplification of a first and a second reference sample; receiving amplification data from amplification of a biological sample; determining an efficiency from the received amplification data from amplification of the first and second reference sample; determining a relative PCR efficiency for the biological sample; and determining a quantity of biological material in the biological sample using the relative PCR efficiency. |
地址 |
Carlsbad CA US |