发明名称 Methods and Systems for Quantification Without Standard Curves
摘要 A method for quantitation of biological material in a biological sample is provided. The method includes receiving amplification data from amplification of a first and a second reference sample and receiving amplification data from amplification of a biological sample. The method further includes determining an efficiency from the received amplification data from amplification of the first and second reference sample. The method includes determining a relative PCR efficiency for the biological sample. Next, the method includes determining a quantity of biological material in the biological sample using the relative PCR efficiency.
申请公布号 US2017022552(A1) 申请公布日期 2017.01.26
申请号 US201515113030 申请日期 2015.01.20
申请人 LIFE TECHNOLOGIES CORPORATION 发明人 Gjerstad Carmen;Dong Shoulian
分类号 C12Q1/68 主分类号 C12Q1/68
代理机构 代理人
主权项 1. A method for quantitation of biological material in a biological sample, the method comprising: receiving amplification data from amplification of a first and a second reference sample; receiving amplification data from amplification of a biological sample; determining an efficiency from the received amplification data from amplification of the first and second reference sample; determining a relative PCR efficiency for the biological sample; and determining a quantity of biological material in the biological sample using the relative PCR efficiency.
地址 Carlsbad CA US