MULTIPLE TAGGING OF INDIVIDUAL LONG DNA FRAGMENTS

摘要

This disclosure provides methods and compositions for tagging long fragments of a target nucleic acid for sequencing and analyzing the resulting sequence information in order to reduce errors and perform haplotype phasing, for example.

主权项

1. A method of sequencing a target nucleic acid comprising:
(a) combining in a single reaction vessel (i) a plurality of long fragments of the target nucleic acid, and (ii) a population of polynucleotides, wherein each polynucleotide comprises a tag and a majority of the polynucleotides comprise a different tag; (b) introducing into a majority of the long fragments tag-containing sequences from said population of polynucleotides to produced tagged long fragments, wherein each of the tagged long fragments comprises a plurality of the tag-containing sequences at a selected average spacing, and each tag-containing sequence comprises a tag; (c) producing a plurality of subfragments from each tagged long fragment, wherein each subfragment comprises one or more tags; (d) sequencing the subfragments to produce a plurality of sequence reads; (e) assign a majority of the sequence read to corresponding long fragments; and assembling the sequence reads to produce an assembled sequence of the target