PROCESS FOR BONE TISSUE DECELLULARIZATION

摘要

A process for preparing decellularized isolated bone tissue samples, comprises the steps of a) providing an isolated bone tissue sample, b) treating said sample by immersions in: a solution comprising at least one chelating agent, a solution comprising at least one anionic detergent, a solution comprising at least one non-ionic detergent, a solution comprising at least one enzyme, a solution comprising at least one antibiotic. Decellularized bone tissue samples as obtainable by a process according to the invention can be used as grafts.

主权项

1. A process for preparing a decellularized isolated bone tissue sample, comprising the steps of:
a. providing an isolated bone tissue sample, and b. treating said isolated bone tissue sample with:
i. a solution comprising at least one chelating agent,ii. a solution comprising at least one anionic detergent,iii. a solution comprising at least one non-ionic detergent,iv. a solution comprising at least one enzyme, andv. a solution comprising at least one antibiotic, wherein said steps b.i. to b.v. are carried out by immersion of said isolated bone tissue sample in said respective solutions.