Isolation and functional identification of cells

摘要

The invention relates to a method for tagging, identifying and isolating a target cell, wherein the cell is contacted with a capture compound comprising a small molecule moiety, for example a pharmaceutical drug molecule, capable of selectively binding with the cell surface of the target cell, and a function that is capable to covalently or quasi-covalently bind to a surface as a marker or tagging function. In some embodiments the capture compound also includes a photoactivatable reactivity function. The capture compound attaches to a particle that mediates identification or isolation of the tagged cell.

主权项

1. A surface for isolating a target cell, comprising the surface of a magnetic particle, the surface of a gold particle, or the surface of a fibre material, and wherein the surface is characterized by having attached to said surface covalently or quasi-covalently
a) a small molecule moiety of below 3500 u molecular mass, which is a ligand for a matrix metalloproteinase, and b) a photo-activatable reactivity function.