Optical apparatus and method for fluorescence measurement of analytes comprising backscattering detection

摘要

An optical apparatus comprises an ultraviolet light source (100) configured to transmit the ultraviolet light to a sample (102), one or more wavelength dependent beam splitters (104) and at least two separate detectors (106, 108). Each beam splitter (104) receives, from the sample (102), a band of excitating ultraviolet light and at least one band of fluorescence associated with an interaction between the excitating ultraviolet light and the sample (102) in the optical path through the sample (102), directs the band of excitating ultraviolet light passed through the sample (102) towards a first detector (106), and directs the at least one band of the fluorescence towards at least one separate detector (108). The first detector (106) and the at least one separate detector (108) are simultaneously configured to form electrical signals carrying information on powers of the bands of the ultraviolet light and the fluorescence, respectively. The signal processing unit (110) configured to measure the at least one property of the sample (102) on the basis of a signal from the first detector (106) and each signal from the at least one separate detector (108).

主权项

1. An optical apparatus, characterized in that the apparatus comprises an ultraviolet light source configured to transmit the ultraviolet light to a sample, one or more wavelength dependent beam splitters, at least one backscattering beam splitter, at least two back scattering detectors, and at least two separate detectors, wherein:
each beam splitter is configured to receive, from the sample, a band of excitating ultraviolet light and at least one band of fluorescence associated with an interaction between the excitating ultraviolet light and the sample in the optical path through the sample, to direct the band of excitating ultraviolet light passed through the sample towards a first detector, and to direct the at least one band of the fluorescence towards at least one separate detector; the first detector and the at least one separate detector are simultaneously configured to form electrical signals carrying information on powers of the bands of the ultraviolet light and the fluorescence, respectively; the at least one backscattering beam splitter is configured to receive ultraviolet light and fluorescence scattered backwards from the sample from a direction different from parallel to the transmitted ultraviolet light, and direct them to different back scattering detectors; the at least two back scattering detectors are configured to separately detect ultraviolet light and fluorescence scattered backwards from the sample; and a signal processing unit is configured to measure the at least one property of the sample on the basis of a signal from the first detector and each signal from the at least one separate detector, and to calibrate the measurement of the at least one property on the basis of the back scattering for compensating additional scattering in the optical path or in the sample.