发明名称 |
Fucosylation-deficient cells |
摘要 |
An isolated nucleic acid encoding an FX protein having a serine at position 79, a lysine at position 90, a leucine at position 136, an arginine at position 211, a serine at position 289, and a combination thereof is provided. Cells having a gene encoding a modified FX protein are provided, wherein the cells exhibit a reduced ability to fucosylate a glycoprotein at a first temperature, but exhibit the ability to fucosylate the glycoprotein at a second temperature. Methods and compositions for making glycoproteins with reduced fucosylation are provided. |
申请公布号 |
US9550823(B2) |
申请公布日期 |
2017.01.24 |
申请号 |
US201514963178 |
申请日期 |
2015.12.08 |
申请人 |
REGENERON PHARMACEUTICALS, INC. |
发明人 |
Chen Gang;Burakov Darya;Fandl James P. |
分类号 |
C12P21/04;C12N9/02;C12N15/00;C12N1/20;C07H21/04;C07K16/00;C07K16/28;C12N9/04;C12P21/00 |
主分类号 |
C12P21/04 |
代理机构 |
Scully, Scott, Murphy & Presser, P.C. |
代理人 |
Scully, Scott, Murphy & Presser, P.C. ;Johnson Mary |
主权项 |
1. A method for making a glycoprotein with reduced fucosylation, comprising:
culturing a mammalian cell that expresses a GDP-4-keto-6-deoxymannose-3,5-epimerase-4-reductase (FX) protein comprising the amino acid sequence of SEQ ID NO: 1, except for an amino acid substitution of L289S, at a temperature of about 37° C. and in the absence of an external fucose source, wherein the cell expresses a glycoprotein; and, isolating the glycoprotein from the culture. |
地址 |
Tarrytown NY US |