CELL CULTURE METHOD AND CELL CULTURE SYSTEM

摘要

A cell culture method in which cells are cultured by using a plurality of culture containers, each including one port for transferring contents with other containers, includes accommodating a cell suspension containing the cells and a culture medium within a first culture container among the plurality of culture containers, connecting a culture medium supply container that supplies a culture medium to the plurality of culture containers and the first culture container with a tube, and other culture containers among the plurality of culture containers are connected in sequence to the first culture container by a tube, starting cell culture in the first culture container, and after completion of the culture in the first culture container, transferring a cell suspension that contains cultured cells from the first culture container to a second culture container connected to the first culture container.

主权项

1. A cell culture method in which cells are cultured by using a plurality of culture containers, wherein each of the plurality of culture containers comprises one port for transferring contents with other containers, and the method comprises:
accommodating a cell suspension containing the cells and a culture medium within a first culture container among the plurality of culture containers, connecting a culture medium supply container that supplies a culture medium to the plurality of culture containers and the first culture container by means of a tube, and other culture containers among the plurality of culture containers are connected in sequence to the first culture container by means of a tube, starting cell culture in the first culture container, after completion of the culture in the first culture container, transferring a cell suspension that contains cultured cells from the first culture container to a second culture container connected to the first culture container, and subsequently, supplying a culture medium from the culture medium supply container to the first culture container, and after the lapse of a prescribed period of time, supplying the culture medium from the first culture container to the second culture container to conduct cell culture in the second culture container, and after completion of the culture in the second culture container, if there is a subsequent culture container connected to the culture container in which the culture has been completed, repeatedly conducting transfer of the cell suspension to the subsequent culture container, supply of a culture medium to the culture container in which the culture has been completed, supply of the culture medium from the culture container in which the culture has been completed to the subsequent culture container, and culture of the cells in the subsequent culture container, until there is no subsequent culture container.