| 发明名称 |
Downstream Processing of an Alkaline Phosphatase |
| 摘要 |
The invention relates to the field of downstream processing (DSP) of an alkaline phosphatase (AP). More specifically, it relates to a method for reducing host cell protein content in a composition comprising AP. The invention further relates to a composition comprising an AP and a reduced content of a host cell protein. |
| 申请公布号 |
US2017009217(A1) |
申请公布日期 |
2017.01.12 |
| 申请号 |
US201515113699 |
申请日期 |
2015.01.26 |
| 申请人 |
AM-PHARMA B.V. |
发明人 |
JONK Luigi Johannes Cornelius;CONNER Stephen Edward;VAN DEN BERG Erik Jan;VAN ELSAS Andrea;SHUKLA Abhinav Alok;HORNE Heather Bethea;COOK Susan;KELLY Timothy Martin;DOWLING Victoria Anne;RAMAROSON Mialy Fanjamalala |
| 分类号 |
C12N9/16;A61K47/10;A61K47/22;A61K47/26;A61K38/46;A61K47/12 |
主分类号 |
C12N9/16 |
| 代理机构 |
|
代理人 |
|
| 主权项 |
1. A method for producing a composition comprising an isolated alkaline phosphatase (AP) and comprising less than 100 ppm host cell protein (HCP), the method comprising a first purification step comprising the steps of:
providing a solid phase comprising a ligand having the formula:wherein R denotes a spacer molecule which links the ligand to the solid phase,
contacting said ligand with a composition comprising an isolated AP having at least 90% sequence identity with SEQ ID NO: 1 and a HCP; performing several wash steps, wherein at least one wash step is performed using a wash buffer comprising between 20-100 mM arginine (Arg), between 0.5-2 M Urea, or between 5-15% ethylene glycol, or any combination thereof; and obtaining AP using an elution buffer capable of detaching at least part of said AP from the ligand. |
| 地址 |
Bunnik NL |
