Methods of biosynthesizing bacterial extracellular galactomannan polysaccharides and subunits thereof for use in subterranean formation operations

摘要

Methods of biosynthesizing bacterial extracellular galactomannan polysaccharides for use in subterranean formation operations such as drilling operations, fracturing operations, or gravel packing operations including providing a feedstock comprising mannose and galactose; providing bacteria effective at fermenting mannose and galactose; introducing the bacteria to the feedstock; and fermenting the bacteria so as to produce an extracellular galactomannan polysaccharide.

主权项

1. A method comprising:
providing a feedstock comprising a carbon source in an amount of about 10% to about 50% by volume of the feedstock and having a water activity of about 0.75 to about 1, wherein the feedstock further comprises mannose and galactose; providing bacteria capable of fermenting mannose and galactose, wherein the mannose and galactose combined are present in the feedstock in an amount of about 0.1% to about 10% by volume of the feedstock; introducing the bacteria to the feedstock; and fermenting the feedstock so as to produce an extracellular galactomannan polysaccharide, wherein about 50% of a dry weight of the bacteria after fermentation comprises the carbon source.