Pump and injector for liquid chromatography

摘要

A combined dual pump-injector valve utilizing a single piece of material to house the barrel for each of the two piston-based pumps and to provide the stator of the associated valve, thus eliminating any need for connections between the pumps and the valve, and therefore eliminating the potential for high-pressure leaks or pressure reductions. The combined dual pump-injector valve permits injection of nanoliter-sized samples into a chromatographic column such that complete analyses can be completed with microliters of mobile phase with nanoliters of a sample.

主权项

1. An integral nano-scale pump and injection valve system (100) for high performance liquid chromatography comprising:
an integrated barrel-stator (110), said integrated barrel-stator (110) having a first elongate barrel (116), a second elongate barrel (116B), and a stator (210) integrally formed of a single piece of monolithic material, said integrated barrel-stator (110) having an integrated barrel-stator first section (220) and an integrated stator barrel second section (222),
said stator (210) having a stator first side (240),said first elongate barrel (116) having a first elongate barrel first open end (430) in said integrated barrel-stator first section (220) and a second open end (2704) at said integrated stator barrel-stator second section (222), and a sidewall (2708) defining a first interior chamber (208) adapted to receive a supply of fluid at second open end (2704),said first elongate barrel second open end (2704) at said stator first side (240) and providing a second stator orifice (2814),said second elongate barrel (116B) having a second elongate barrel first open end (430B) in said integrated stator barrel first section (220) and a second elongate barrel second open end (2704) at said integrated barrel-stator second section (222), and a second elongate barrel sidewall (2708B) defining an second interior chamber (208B) adapted to receive a supply of fluid at first open end (2704B),said second elongate barrel second open end (2704B) at said stator first side (240) and providing a first stator orifice (2812),said stator (210) having a first stator fluid supply port (2806) for communication with a liquid supply and having a second stator fluid supply port (2810) for communication with a liquid supply,said stator (210) having a third stator port (2804) and having a fourth stator port (2808),said stator (210) in contact at a surface of a stator lace (2802) with a surface of a rotor face (602) of a rotor (214), said stator (210) adapted to communicate with said rotor (214) at said first stator fluid supply port (2806) and adapted to communicate with said rotor (214) at said second stator fluid supply port (2810), said first stator orifice (2812) and said second stator orifice (2814) being non-overlapping; a first longitudinal plunger (206) slidably disposed within said first interior chamber (208), said first longitudinal plunger (206) being of substantially uniform cross section, a second longitudinal plunger (206B) slidably disposed within said second interior chamber (208B), said second longitudinal plunger (206B) being of substantially uniform cross section, said rotor (214) having a first channel (604) in said rotor face (602) and a second channel (612) in said rotor face (602), said rotor (214) rotable with respect to said stator (210) about a centerpoint of said stator (210) between a load position (202) and an injection position (302),
said load position (202) defined by said second stator orifice (2814) in communication with said first stator fluid supply port (2806) via said first channel (2904) and said first stator orifice (2812) in communication with said second stator fluid supply port (2810) via said second channel (2906), andsaid injection (302) position defined by said second stator orifice (2814) in communication with said third stator port (2804) via said first channel (2904) and said first stator orifice (2812) in communication with said fourth stator port (2808) via said second channel (2906).