METHODS AND ASSAYS FOR DETERMINING REDUCED BRCA1 PATHWAY FUNCTION IN A CANCER CELL

摘要

Provided herein are methods and assays that can be used to predict whether a subject will or will not respond to a cancer treatment comprising a PARP inhibitor and a DNA damage-inducing chemotherapeutic.

主权项

1. A method of treating cancer in a subject, the method comprising:
(a) assaying a biological sample comprising a cancer cell obtained from a subject having cancer or suspected of having cancer for the expression of a combination of at least the following markers: EZR, SOX4, EMG1, NARS, SNRNP70, CLTA, EIF3B, EIF3E, UBAP2L, SF3A3, HK1, CKAP4, NOLC1, HCFC1, PPM1F, BMS1, AIMP2, POLG, MCM2, U2AF1, MOGS, SKIV2L, CCND1, MLEC, RARS, SLC7A5, MANF, RAE1, CHD4, BYSL, GTF3C2, TRAP1, KIAA0114, CDC25B, SF3B2, SMARCA4, DDX11, LMNB2, DHCR24, SAFB, CTPS, DUSP1, DDX10, CDC123, NDRG1, EIF2B2, FAM38A, SEC24C, RABEPK, FGFR3, SSRP1, and SLC1A3; (b) normalizing the expression data for each of the markers to a control, and (c) administering to the subject a therapeutically effective amount of a poly ADP ribose polymerase (PARP) inhibitor and a DNA damage-inducing chemotherapeutic agent when expression of the combination of markers is increased compared to a reference value and not administering a PARP inhibitor and a DNA damage-inducing chemotherapeutic agent when the expression of the combination of markers is not changed or decreased compared to a reference value.