发明名称 |
RNA PREPARATIONS COMPRISING PURIFIED MODIFIED RNA FOR REPROGRAMMING CELLS |
摘要 |
The present invention provides compositions and methods for reprogramming somatic cells using purified RNA preparations comprising single-strand mRNA encoding an iPS cell induction factor. The purified RNA preparations are preferably substantially free of RNA contaminant molecules that: i) would activate an immune response in the somatic cells, ii) would decrease expression of the single-stranded mRNA in the somatic cells, and/or iii) active RNA sensors in the somatic cells. In certain embodiments, the purified RNA preparations are substantially free of partial mRNAs, double-stranded RNAs, un-capped RNA molecules, and/or single-stranded run-on mRNAs. |
申请公布号 |
EP3112467(A1) |
申请公布日期 |
2017.01.04 |
申请号 |
EP20160181434 |
申请日期 |
2010.12.07 |
申请人 |
The Trustees of the University of Pennsylvania |
发明人 |
Dahl, Gary;Meis, Judith;Person, Anthony;Jendrisak, Jerome;Karikó, Katalin;Weissman, Drew |
分类号 |
C12N15/113;C12N5/02;C12N5/071 |
主分类号 |
C12N15/113 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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