Marker for determining effects of anti-c-Met antibody and method of determining effects of anti-c-Met antibody using the marker

摘要

There are provided a composition for determining the efficacy of a c-Met antibody including marker genes and a method for determining the efficacy of a c-Met antibody using the marker genes.

主权项

1. A method for determining the anticancer efficacy of an anti-c-Met antibody, comprising:
treating a cell sample with the anti-c-Met antibody, wherein the anti c-Met antibody specifically binds to an epitope comprising 5 to 19 contiguous amino acids of SEQ ID NO: 71 comprising the amino acid sequence of SEQ ID NO: 73; measuring the expression of one or more genes selected from the group consisting of a TNFRSF21 gene, a CASP10 gene, a TP53 gene, a BCL2 gene, and a BCL2L1 gene in the cell sample treated with the anti-c-Met antibody; and comparing the expression of the one or more genes with a control, wherein when the control is a cell sample that has not been treated with the anti-c-Met antibody and the expression of one or more genes selected from the group consisting of the TNFRSF21 gene, the CASP10 gene, and the TP53 gene in the cell sample treated with the anti-c-Met antibody is increased relative to the control depending on the treatment concentration of the anti-c-Met antibody, or the expression of one or more genes selected from the group consisting of the BCL2 gene and the BCL2L1 gene is decreased relative to the control depending on the treatment concentration of the anti-c-Met antibody, then the anti-c-Met antibody exhibits anticancer efficacy on the cell sample or a patient from which the cell sample is obtained, and wherein the anti-c-Met antibody comprises: (i) a heavy chain variable region comprising (a) a CDR-H1 comprising the amino acid sequence of SEQ ID NO: 4; (b) a CDR-H2 comprising the amino acid sequence of SEQ ID NO: 5, the amino acid sequence of SEQ ID NO: 2, or an amino acid sequence comprising 8-19 consecutive amino acids comprising amino acid residues from the 3rd to 10th positions of the amino acid sequence of SEQ ID NO: 2; and (c) a CDR-H3 comprising the amino acid sequence of SEQ ID NO: 6, the amino acid sequence of SEQ ID NO: 85, or an amino acid sequence comprising 6-13 consecutive amino acids comprising amino acid residues from the 1st to 6th positions of the amino acid sequence of SEQ ID NO: 85; and (ii) a light chain variable region comprising (a) a CDR-L1 comprising the amino acid sequence of SEQ ID NO: 7, (b) a CDR-L2 comprising the amino acid sequence of SEQ ID NO: 8, and (c) a CDR-L3 comprising SEQ ID NO: 13, SEQ ID NO: 14, SEQ ID NO: 15, or SEQ ID NO: 16.