发明名称 |
AN OPTICAL INSTRUMENT MONITORING DNA POLYMERASE CHAIN REACTIONS |
摘要 |
An optical instrument (A) for monitoring polymerase chain reaction replication of DNA may include a thermal cycler block (1c) for holding a plurality of vials (1b), each containing a suspension of ingredients that include a fluorescent primary dye. The instrument may include a light source (11) for emitting a source beam, a first means (7) disposed to be receptive of the source beam, a primary focusing means (3) disposed to focus the excitation beam simultaneously into a plurality of suspensions and being receptive of and passing emission beams, a second means (8) disposed to be receptive of emission beams, an emission focusing means (10) for focusing emission beams, a detector, and a processing means for computing concentration of DNA. The optical system being arranged without a beam splitter (6) but with one or more folding mirrors (5), and with excitation and emission beams being on slightly different optical paths angularly. |
申请公布号 |
EP3093649(A2) |
申请公布日期 |
2016.11.16 |
申请号 |
EP20160172036 |
申请日期 |
1999.05.17 |
申请人 |
LIFE TECHNOLOGIES CORPORATION |
发明人 |
GAMBINI, MICHAEL R.;ATWOOD, JOHN G.;YOUNG, EUGENE F.;LAKATOS, EDWARD J.;CERRONE, ANTHONY L. |
分类号 |
G01N21/03;B01L7/00;C12N15/09;C12Q1/68;G01N21/25;G01N21/64;G01N21/78 |
主分类号 |
G01N21/03 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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