发明名称 |
SYNTHESIS OF POOLS OF PROBES BY PRIMER EXTENSION |
摘要 |
Provided herein is a method for making a pool of probes by primer extension. In certain embodiments, the method comprises hybridizing a first population of oligonucleotides comprising a top strand sequence having the following formula V1-B-3′ with a second population of oligonucleotides comprising a bottom strand sequence having the following formula V2′-B′-3′ to provide a population of duplexes. After hybridizing, the 3′ ends of the oligonucleotides in the duplexes are extended to produce a population of double stranded products comprising a top strand sequence having the following formula V1-B-V2, where V2 is complementary to V2′. |
申请公布号 |
EP2964777(A4) |
申请公布日期 |
2016.10.26 |
申请号 |
EP20130876980 |
申请日期 |
2013.03.05 |
申请人 |
AGILENT TECHNOLOGIES, INC. |
发明人 |
RUVOLO, MICHAEL;LEPROUST, EMILY, MARINE |
分类号 |
C12Q1/68;C12N15/11 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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