RECOMBINANT DNA ELEMENTS FOR THE EXPRESSION OF RECOMBINANT PROTEINS IN INSECTS

摘要

Reagents and methods are provided that allow for an improved expression of a recombinant protein in an insect More specifically, the introduction of recombinant DNA elements into an insect larva allows for the increased expression of a recombinant protein, an improvement of the correct folding of said protein and an increase in the survival rate after infection of the insect These recombinant DNA elements can be introduced, for example, into insect larvae via a recombinant baculovirus, which has incorporated said elements. The recombinant DNA elements include nucleic acids encoding transcriptional regulators, such as IE-0 and lE-1, transcriptional enhancer elements, such as the homologous region (hr) and promoters.