发明名称 |
METHOD FOR DISTINGUISHING TARGET BASE SEQUENCE |
摘要 |
According to the present invention, a method for identifying a base sequence accompanying competitive hybridization is provided, wherein a nucleic acid having a target base sequence can be identified in an extremely short period of time as compared with conventional methods while maintaining the accuracy of identifying differences in base sequences. The method of the present invention comprises a thermal denaturation step for subjecting a sample double-stranded nucleic acid and a reference double-stranded nucleic acid containing the same base sequence as a target base sequence to thermal denaturation treatment in a single reaction solution, a temperature lowering step for carrying out competitive hybridization between the sample double-stranded nucleic acid and the reference double-stranded nucleic acid by lowering the temperature of the reaction solution after the thermal denaturation step, a measurement step for measuring a double-stranded nucleic acid formed by a nucleic acid strand that composed the reference double-stranded nucleic acid and a nucleic acid strand that composed the sample double-stranded nucleic acid, and an identification step for identifying identity between the reference double-stranded nucleic acid and the sample double-stranded nucleic acid based on measurement results obtained from the measurement step; wherein, the temperature lowering step is carried out in the presence of a cationic comb-type polymer. |
申请公布号 |
EP2554680(B1) |
申请公布日期 |
2016.10.12 |
申请号 |
EP20110762732 |
申请日期 |
2011.03.25 |
申请人 |
TOPPAN PRINTING CO., LTD.;KYUSHU UNIVERSITY, NATIONAL UNIVERSITY CORPORATION |
发明人 |
KITANO, SHIRO;YAMANE, AKIO;MARUYAMA, ATSUSHI |
分类号 |
C12Q1/68;C12N15/09;G01N21/78;G01N33/53;G01N33/533 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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