POLYPEPTIDE COMPOSITION AND CULTURE METHOD FOR PLURIPOTENT STEM CELL USING SAME

摘要

Provided are a polypeptide composition, which can induce a pluripotent stem cell culturing property, particularly, an excellent cell growth ability, and a culture method for pluripotent stem cells using the polypeptide composition. The polypeptide composition contains a predetermined polypeptide including an amino acid sequence of human vitronectin or an amino acid sequence of a predetermined first region derived from human vitronectin. In the polypeptide composition, the amount of a multimeric polypeptide, which is composed of two or more monomers held together by intermolecular cross-linking via cysteine residues included in the first region, is equal to or less than 20% by mass of a total mass of polypeptides contained in the composition. The culture method for pluripotent stem cells includes culturing pluripotent stem cells in the presence of the polypeptide composition. Also provided is a culture vessel including a support which has a cell culture surface and the polypeptide contained in the polypeptide composition disposed on the cell culture surface of the support.