发明名称 |
METHOD FOR THE PRODUCTION OF A GLYCOSYLATED IMMUNOGLOBULIN |
摘要 |
Herein is reported a method for the production of an immunoglobulin comprising the following steps: a) providing a eukaryotic cell comprising a nucleic acid encoding the immunoglobulin, b) cultivating the eukaryotic cell in a cultivation medium wherein the amount of glucose available in the cultivation medium per time unit is kept constant and limited to less than 80% of the amount that could maximally be utilized by the cells in the cultivation medium per time unit, and c) recovering the immunoglobulin from the culture. |
申请公布号 |
US2016186228(A1) |
申请公布日期 |
2016.06.30 |
申请号 |
US201514844570 |
申请日期 |
2015.09.03 |
申请人 |
Hoffmann-La Roche Inc. ;Chugai Seiyaku Kabushiki Kaisha |
发明人 |
Franze Reinhard;Hirashima Chikashi;Link Thomas;Takagi Yoshinori;Takuma Shinya;Tsuda Yuriko |
分类号 |
C12P21/00;C07K16/28 |
主分类号 |
C12P21/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
Little Falls NJ US |