发明名称 |
GENETICALLY ENCODED PROBE FOR QUANTIFICATION OF PYRUVATE CONCENTRATION AND METHODS OF USING THE SAME |
摘要 |
The present invention relates to a new optical tool for detecting and quantifying pyruvate in samples, in tissues and in cellular and subcellular compartments, with high spatial and temporal resolution, this is a Forster Resonance Energy Transfer (FRET)-based pyruvate sensor comprising a bacterial PdhR transcription factor between any suitable donor and acceptor fluorescent proteins moieties. The invention also relates to methods of use of this novel optical tool for the quantification of the activity of pyruvate transporters, for the quantification of the rates of cellular pyruvate production and consumption, and for the direct quantification of the rate of mitochondrial pyruvate consumption in intact cells. |
申请公布号 |
EP3036528(A1) |
申请公布日期 |
2016.06.29 |
申请号 |
EP20130891824 |
申请日期 |
2013.08.20 |
申请人 |
CENTRO DE ESTUDIOS CIENTIFICOS DE VALDIVIA |
发明人 |
BARROS, LUIS FELIPE;CEBALLO, SEBASTIÁN;SAN MARTÍN, ALEJANDRO |
分类号 |
G01N21/64;G01N33/53 |
主分类号 |
G01N21/64 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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