SYNTHETIC NUCLEIC ACIDS FOR POLYMERIZATION REACTIONS
摘要
Compositions and methods are provided for inhibiting a polymerase from replicating non target DNA at a temperature below the amplification reaction temperature. The inhibitor is a synthetic nucleic acid which is single stranded but folds to form at least one double stranded region designed to melt at a temperature which is lower than the amplification reaction temperature, and at least one single stranded region where the single stranded region at the 5' end contains at least one uracil or inosine and optionally a sequence at the 3' end contains one or more derivative nucleotide or linkages.
申请公布号
EP2836603(A4)
申请公布日期
2016.06.29
申请号
EP20130775998
申请日期
2013.04.04
申请人
NEW ENGLAND BIOLABS, INC.
发明人
JOHNSON, DONALD;EVANS, THOMAS, C.;GREENOUGH, LUCIA;ONG, JENNIFER