| 发明名称 |
OLIGOSACCHARIDE COMPOSITIONS, GLYCOPROTEINS AND METHODS TO PRODUCE THE SAME IN PROKARYOTES |
| 摘要 |
Disclosed are methods and compositions to produce various oligosaccharide compositions and glycoproteins. Prokaryotic hosts cells are cultured under conditions effective to produce human-like e.g., high-mannose, hybrid and complex glycosylation patterns by introducing glycosylation pathways into the host cells. |
| 申请公布号 |
US2016177355(A1) |
申请公布日期 |
2016.06.23 |
| 申请号 |
US201414775859 |
申请日期 |
2014.03.14 |
| 申请人 |
FISHER Adam C.;MERRITT Judith H.;HAMILTON Brian S.;VALDERRAMA-RINCO Juan D.;DELISA Metthew P.;GLYCOBIA, INC. |
发明人 |
Fisher Adam C;Merritt Judith H;Hamilton Brian S;Valderrama-Rincon Juan D;DeLisa Matthew P |
| 分类号 |
C12P19/04;C07K14/245;C07K14/605;C12P21/00;C12N15/70;C12N15/52;C12N9/10;C07K16/00;A61K39/108 |
主分类号 |
C12P19/04 |
| 代理机构 |
|
代理人 |
|
| 主权项 |
1. A method of producing an oligosaccharide composition, said method comprising:
culturing a recombinant prokaryotic host cell that produces an oligosaccharide composition having a terminal mannose residue to express one or more N-acetylglucosaminyl transferase enzyme activity (EC 2.4.1.101; EC 2.4.1.143; EC 2.4.1.145; EC 2.4.1.155; EC 2.4.1.201) that catalyzes the transfer of a UDP-GlcNAc residue onto said terminal mannose residue, said culturing step carried out under conditions effective to produce an oligosaccharide composition having a terminal GlcNAc residue. |
| 地址 |
Ithaca NY US |
