MAGNETIC SEPARATION OF LIPOPROTEINS USING DEXTRAN SULFATE

摘要

The invention provides apparatus and methods of preparation of lipoproteins from a biological sample, including HDL, LDL, Lp(a), IDL, and VLDL, for diagnostic purposes utilizing differential charged-particle mobility analysis methods. Further provided are methods for analyzing the size distribution of lipoproteins by differential charged-particle mobility, which lipoproteins are prepared by methods of the invention. Further provided are methods for assessing lipid-related health risk, cardiovascular condition, risk of cardiovascular disease, and responsiveness to a therapeutic intervention, which methods utilize lipoprotein size distributions determined by methods of the invention.

主权项

1. A method for purifying lipoproteins suitable for differential charged-particle mobility analysis of lipoprotein class and subclass, said method comprising:
(a) adding one or more cations and dextran sulfate to a sample to form a precipitation mixture; (b) contacting the precipitation mixture with an insoluble solid support, wherein one or more classes or subclasses of lipoproteins from said sample precipitate from said mixture onto said solid support; (c) separating said precipitated lipoproteins on said solid support from said mixture; and (d) removing said precipitated lipoproteins from said solid support;
wherein said solid support binds precipitated lipoproteins without an immunoaffinity agent; andwherein said dextran sulfate is not biotinylated and said precipitated lipoproteins are not bound to said solid support by streptavidin.