发明名称 |
TARGET REGION ENRICHMENT METHOD BASED ON MULTIPLEX PCR, AND REAGENT |
摘要 |
Provided are a target region enrichment method based on multiplex PCR, and a reagent, the method comprising: connecting a first linker and a second linker respectively at two ends of a nucleic acid segment containing target regions to be enriched so as to obtain a linker-connected product; performing a PCR amplification on the linker-connected product using a first primer specifically bound to the first linker and a second primer specifically bound to the second linker to obtain an amplified product, the first primer or the second primer having a first affinity label; capturing a single strand having the first affinity label in the amplified product using a solid phase carrier; performing single primer linear amplification using a third primer with the captured single strand as a template; performing exponential amplification using the third primer and the first primer, with the linearly amplified product as the template, to obtain a product containing the target regions. |
申请公布号 |
WO2016095736(A1) |
申请公布日期 |
2016.06.23 |
申请号 |
WO2015CN96790 |
申请日期 |
2015.12.09 |
申请人 |
BGI SHENZHEN;BGI SHENZHEN CO., LIMITED |
发明人 |
GUO, JING;GUO, RONGRONG;LI, MEIYAN;GENG, CHUNYU;JIANG, HUI |
分类号 |
C12N15/11 |
主分类号 |
C12N15/11 |
代理机构 |
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代理人 |
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地址 |
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