发明名称 |
METHOD FOR PRODUCING, ISOLATING AND PURIFYING RECOMBINANT HUMAN ANTITRYPTASE (OSRAAT) FROM RICE SEEDS |
摘要 |
Provided in the present invention are a rice genetic codon-optimized OsrAAT gene, related vector and a method for expression of OsrAAT in transgenic rice seeds by using the vector and a method for isolating and purifying OsrAAT. A transgenic rice seed expressing OsrAAT is prepared by the endosperm-specific expression vector. The purity of OsrAAT isolated and purified from transgenic rice grains reached 97% with HPLC grade and the yield of OsrAAT was up to 18.89 ± 3.19% of total soluble proteins, thus corresponding that 0.366 g of OsrAAT per kilogram can be produced from brown rice. |
申请公布号 |
EP2918680(A4) |
申请公布日期 |
2016.06.22 |
申请号 |
EP20130853496 |
申请日期 |
2013.04.26 |
申请人 |
WUHAN HEALTHGEN BIOTECHNOLOGY CORP |
发明人 |
YANG, DAICHANG;SHI, QIANNI;ZHANG, LIPING |
分类号 |
C12N15/15;A01H5/10;C07K1/18;C07K14/81;C12N15/63;C12N15/82 |
主分类号 |
C12N15/15 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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