发明名称 |
METHODS FOR GENERATING ENGINEERED ENZYMES |
摘要 |
Provided are improved methods for identifying the substrate recognition specificity or activity of a protease, convertase (sortase), or kinase. In some embodiments, methods are provided for identifying the endogenous protease or convertase cleaving patterns (e.g., "cleaveOme") inside the secretory pathway of a living cell. Select embodiments involve aspects of yeast endoplasmic reticulum sequestration screening and next generation sequencing. Methods of producing polypeptides in Kex2 knockout yeast are also provided. |
申请公布号 |
WO2016061199(A3) |
申请公布日期 |
2016.06.09 |
申请号 |
WO2015US55494 |
申请日期 |
2015.10.14 |
申请人 |
RESEARCH DEVELOPMENT FOUNDATION |
发明人 |
IVERSON, BRENT;MAREK, PETER;TAFT, JOSEPH |
分类号 |
C12Q1/37;C12N15/10;C12N15/81;C12Q1/48 |
主分类号 |
C12Q1/37 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|