IMMUNOGLOBULIN FC CONJUGATE WHICH MAINTAINS BINDING AFFINITY OF IMMUNOGLOBULIN FC FRAGMENT TO FCRN

摘要

The present invention relates to a physiologically active polypeptide-immunoglobulin Fc fragment conjugate, which comprises a physiologically active polypeptide linked via a non-peptidyl linker to an immunoglobulin Fc fragment having an FcRn-binding region and maintains the intrinsic binding affinity of the immunoglobulin Fc fragment, a method for preparing the conjugate, a method of maintaining the intrinsic binding affinity of the conjugate for FcRn, and a composition comprising the conjugate, which maintains the intrinsic binding affinity of the immunoglobulin Fc fragment for FcRn.

主权项

1. A physiologically active polypeptide-immunoglobulin Fc fragment conjugate that comprises a physiologically active polypeptide linked via a non-peptidyl linker to an immunoglobulin Fc fragment comprising an FcRn-binding region, wherein a binding ratio of the amount of the conjugate bound to FcRn at pH 6.0 and the amount of the conjugate bound to FcRn at pH 7.4 is within the range of ±6% of a ratio determined by measuring the amounts of the immunoglobulin Fc fragment bound to FcRn under the same conditions as those used for the conjugate.