PRODUCING A NANOPORE FOR SEQUENCING A BIOPOLYMER

摘要

A process for producing at least one nanopore with a predetermined diameter for sequencing a biopolymer is provided herein. This process includes providing at least one electrode and at least two nanoparticles in an intervening space between the electrode and a delimiting component opposite to the electrode. The electrode is coated with an electrically conductive material with resultant mechanical fixing of the at least two nanoparticles in the intervening space, thus producing a fixed porous arrangement. Charging of the electrically conductive material and/or another electrically conductive material to the fixed porous arrangement permits the establishment of a predetermined diameter of at least one pore, such as the formation of the nanopore. A process for sequencing the biopolymer with the aid of a fixed porous arrangement, as well as a corresponding device, are also provided herein.

主权项

1. A method for producing at least one nanopore having a predetermined diameter for sequencing a biopolymer in a chamber of an apparatus, the method comprising:
providing an electrode and at least two nanoparticles in the chamber, wherein the at least two nanoparticles are arranged in an interspace between the electrode and a delimiting component situated opposite the electrode, coating the electrode with at least one electrically conductive material, thereby mechanically fixing the at least two nanoparticles in the interspace, such that a fixed porous arrangement arises in the interspace; and, filling the fixed porous arrangement with the at least one electrically conductive material, thereby setting a diameter of at least one pore of the fixed porous arrangement to a predetermined diameter, such that the nanopore is formed, and/or wherein the nanopore is delimited by the at least two nanoparticles such that the at least two nanoparticles space apart the at least one electrically conductive material arranged on the electrode from the delimiting component or a conductive connection to the delimiting component.