| 摘要 |
PROBLEM TO BE SOLVED: To provide a novel nucleic acid amplification method capable of performing efficient nucleic acid amplification.SOLUTION: A nucleic acid amplification method includes: a process of heating a first region of a nucleic acid amplification reaction container filled with nucleic acid amplification reaction liquid and liquid which has smaller specific gravity than the nucleic acid amplification reaction liquid and does not mix with the nucleic acid amplification reaction liquid, to a first temperature; a process of heating a second region of the nucleic acid amplification reaction container to a second temperature lower than the first temperature; a process of switching a first arrangement in which the first region comes under the second region in a working direction of gravity to a second arrangement in which the second region comes under the first region in a working direction of gravity; and a process of switching the second arrangement to the first arrangement. The nucleic acid amplification reaction liquid contains a probe, and the probe contains artificial nucleic acid.SELECTED DRAWING: None |
